The video is being compared to one that was released by George Bush's campaign during the 1996 election, which was was said to have used racist imagery.
The ad centers on Luis Bracamontes, who was in the country illegally when he killed the officers in California and is now on death row, reports the Sacramento Bee.
The caption reads: "Illegal immigrant, Luis Bracamontes, killed our people!" "This ad, and your full approval of it, will condemn you and your bigoted legacy forever in the annals of America's history books".
Horton was a convicted murderer who went on a crime spree while on furlough from prison under a program supported by Dukakis, then governor of MA.
President Donald Trump knows how upended his administration is going to be if a blue wave does indeed sweep through the country in next Tuesday's midterm elections. The campaign did not immediately respond to a request for comment.
CNN's Stephen Collinson writes this morning that "Trump's web video, while just as shocking as the Horton spot, carries added weight since, unlike its 1988 predecessor, it bears the official endorsement of the leader of the Republican Party-Trump-and is not an outside effort".
The new video blames Democrats for letting Bracamontes into the USA and allowing the undocumented immigrant to stay in the country. Conservative commentator Scott Jennings also admitted to Cuomo that he wasn't surprised by the depravity of the ad, stating, "I don't see anything in this video that I haven't heard from the President consistently for the past couple of years".
Cardenas called Trump "a despicable divider; the worse social poison to afflict our country in decades". His snarling, expletive-filled rant about killing police officers appeared in a little-noticed January 2018 Trump campaign ad.
The video shows a Latino man in court, smiling menacingly.
"The reason Trump will be here is he is nervous because his candidate might lose", Bernie Sanders, the former Democratic presidential candidate and independent senator for Vermont, told students at the University of Central Florida in Orlando. The ad was widely criticised for emphasising that Horton was black.