U.S. Ambassador to the State of Israel David M. Friedman, Treasury Secretary Steven Mnuchin and assistant to the president Jason D. Greenblatt will also attend.
It remains unclear if President Trump will be on hand to officially open the diplomatic headquarters.
Israel occupied east Jerusalem in the 1967 Six-Day War and later annexed it.
In Jerusalem, there were at least three road sign that says the U.S. Embassy in three languages: English, Hebrew and Arabic.
In June 2017, however, like his predecessors, Trump signed a six-month waiver to delay the relocation, which would have complicated U.S. efforts to resume the long-stalled Israeli-Palestinian peace talks.
Trump said last week that he "may" go to Jerusalem for the opening himself.
At the consulate site, mechanical diggers cleared scrubland as workers posted embassy signs along city roads and hung U.S., Israeli and Jerusalem flags from street lights.
Israel claims the entire city as its eternal capital. The Palestinians, however, see East Jerusalem as the capital of their future state.
The worldwide community, including the U.S., does not recognise Israel's jurisdiction and ownership of the city. "The relationship has never been better", Trump said of Israel.
Israeli Prime Minister Benjamin Netanyahu said in April "at least half a dozen" countries were now "seriously discussing" following the USA lead, though he did not identify them.
Senior Palestinian official Saeb Erekat called in a statement Monday for diplomats, "civil society organisations, and religious authorities to boycott the inauguration ceremony" of the Jerusalem embassy.
Trump sparked worldwide outcry when he unilaterally recognized Jerusalem as Israel's capital and vowed to relocate Washington's embassy to the city.
The embassy is expected to open next week.