The planned South America trip was meant to see Trump attend the Summit of the Americas in Lima, the capital city of Peru.
During that trip, Pence met with the presidents of Argentina, Chile, Colombia and Panama to negotiate trade deals and discuss concerns about Venezuelan President Nicolas Maduro's regime, which has been described as increasingly dictatorial by the US and Colombia.
His travel plans changed after a Saturday night attack on the Syrian town of Douma which killed at least 60 people and injured more than 1,000 others.
The earlier US announcement that President Donald Trump will not attend the 8th Summit of the Americas to be held this week here in the Peruvian capital reflects a "low priority" for Latin America on Washington's agenda, said Peruvian analysts.
Syria denies being behind any chemical attack.
White House officials said the decision to miss the summit was made Monday, adding that the raid of the office of Trump attorney Michael Cohen was not a factor in the decision.
"Trump has no positive agenda for Latin America that he could present at the summit". Trump values his European allies and has often said so.
The summit would have been Trump's first trip to the region.
The Acting Secretary will also engage with members of Cuban and Venezuelan independent civil society, regional business leaders, and U.S. Embassy staff.
Several nations, including the United States attended an emergency session of the U.N. Security Council during which U.S. Ambassador to the U.N. Nikki Haley said that Russian Federation was "complicit" in the attack.